› 2fjh

X-ray diffraction
3.1Å resolution

Structure of the B20-4 Fab, a phage derived Fab fragment, in complex with VEGF

Released:
DOI: 10.2210/pdb2fjh/pdb
PDB entry 2fjh coloured by chain, front view.
PDB entry 2fjh coloured by chain, side view.
PDB entry 2fjh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-function studies of two synthetic anti-vascular endothelial growth factor Fabs and comparison with the Avastin Fab.
Fuh G, Wu P, Liang WC, Ultsch M, Lee CV, Moffat B, Wiesmann C
J Biol Chem 281 6625-31 (2006)
PMID: 16373345

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210646 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
N-VEGF Chains: V, W
Molecule details ›
Chains: V, W
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 214-315; Coverage: 26%)
Gene names: VEGF, VEGFA
Sequence domains: VEGF heparin-binding domain
Structure domains: Cystine-knot cytokines
Fab fragment light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab fragment heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 228 amino acids
Theoretical weight: 24.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3112
Unit cell:
a: 165.538Å b: 165.538Å c: 150.548Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.198 0.239
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Protein kinase inhibitors: contributions from structure to clinical compounds.
Johnson LN. (2009)
15 more

8 mentions without citation

A Structural Overview of Vascular Endothelial Growth Factors Pharmacological Ligands: From Macromolecules to Designed Peptidomimetics.
Ye et al. (2021)
7 more