› 2fot

X-ray diffraction
2.45Å resolution

Crystal structure of the complex between calmodulin and alphaII-spectrin

Released:
DOI: 10.2210/pdb2fot/pdb
PDB entry 2fot coloured by chain, front view.
PDB entry 2fot coloured by chain, side view.
PDB entry 2fot coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the calmodulin alphaII-spectrin complex provides insight into the regulation of cell plasticity.
Simonovic M, Zhang Z, Cianci CD, Steitz TA, Morrow JS
J Biol Chem 281 34333-40 (2006)
PMID: 16945920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158582 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P62157 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CAM
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Spectrin alpha chain, non-erythrocytic 1 Chain: C
Molecule details ›
Chain: C
Length: 42 amino acids
Theoretical weight: 4.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13813 (Residues: 1172-1190, 1191-1211; Coverage: 2%)
Gene names: NEAS, SPTA2, SPTAN1

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 44.288Å b: 57.737Å c: 69.753Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.269
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structural diversity of calmodulin binding to its target sites.
Tidow et al. (2013)
5 more

10 mentions without citation

Global analysis of posttranslational protein arginylation.
Wong et al. (2007)
9 more