› 2fp0

X-ray diffraction
2.05Å resolution

human ADP-ribosylhydrolase 3

Released:
DOI: 10.2210/pdb2fp0/pdb
PDB entry 2fp0 coloured by chain, front view.
PDB entry 2fp0 coloured by chain, side view.
PDB entry 2fp0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human ADP-ribosylhydrolase 3 (ARH3) provides insights into the reversibility of protein ADP-ribosylation.
Mueller-Dieckmann C, Kernstock S, Lisurek M, von Kries JP, Haag F, Weiss MS, Koch-Nolte F
Proc Natl Acad Sci U S A 103 15026-31 (2006)
PMID: 17015823

Function and Biology Details

Reaction catalysed: 
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylhydrolase ARH3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NX46 (Residues: 19-363; Coverage: 95%)
Gene names: ADPRHL2, ADPRS, ARH3
Sequence domains: ADP-ribosylglycohydrolase
Structure domains: ADP-ribosylation/Crystallin J1

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 57.33Å b: 60.63Å c: 102.88Å
α: 90° β: 96.43° γ: 90°
R-values:
R R work R free
0.189 0.188 0.231
Expression system: Escherichia coli

Quick links

Citations

39 review citations

The multifaceted roles of PARP1 in DNA repair and chromatin remodelling.
Ray Chaudhuri et al. (2017)
38 more

4 mentions without citation

The structure of human ADP-ribosylhydrolase 3 (ARH3) provides insights into the reversibility of protein ADP-ribosylation.
Mueller-Dieckmann et al. (2006)
3 more