› 2fso

X-ray diffraction
1.83Å resolution

mitogen activated protein kinase p38alpha (D176A) activating mutant

Released:
DOI: 10.2210/pdb2fso/pdb
PDB entry 2fso coloured by chain, front view.
PDB entry 2fso coloured by chain, side view.
PDB entry 2fso coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of p38alpha active mutants reveal conformational changes in L16 loop that induce autophosphorylation and activation.
Diskin R, Lebendiker M, Engelberg D, Livnah O
J Mol Biol 365 66-76 (2007)
PMID: 17059827

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 14 Chain: X
Molecule details ›
Chain: X
Length: 367 amino acids
Theoretical weight: 42.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16539 (Residues: 2-360; Coverage: 100%)
Gene names: CSBP, CSBP1, CSBP2, CSPB1, MAPK14, MXI2, SAPK2A
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 67.86Å b: 69.415Å c: 74.064Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.224
Expression system: Escherichia coli

Quick links

Citations

6 review citations

MAP kinase signalling cascades and transcriptional regulation.
Yang et al. (2013)
5 more

4 mentions without citation

p38α Mitogen-Activated Protein Kinase Is a Druggable Target in Pancreatic Adenocarcinoma.
Yang et al. (2019)
3 more