› 2fyy

X-ray diffraction
1.5Å resolution

The role of T cell receptor alpha genes in directing human MHC restriction

Released:
DOI: 10.2210/pdb2fyy/pdb
PDB entry 2fyy coloured by chain, front view.
PDB entry 2fyy coloured by chain, side view.
PDB entry 2fyy coloured by chain, top view.
Source organisms:
Primary publication:
TCR alpha genes direct MHC restriction in the potent human T cell response to a class I-bound viral epitope.
Miles JJ, Borg NA, Brennan RM, Tynan FE, Kjer-Nielsen L, Silins SL, Bell MJ, Burrows JM, McCluskey J, Rossjohn J, Burrows SR
J Immunol 177 6804-14 (2006)
PMID: 17082594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-127599 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O19626 (Residues: 25-300; Coverage: 93%)
Gene name: B-3501
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Epstein-Barr nuclear antigen 1 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03211 (Residues: 407-417; Coverage: 2%)
Gene names: BKRF1, EBNA1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 51.045Å b: 81.783Å c: 110.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.236
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

12 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
11 more

8 mentions without citation

Cross-reactive CD8+ T-cell immunity between the pandemic H1N1-2009 and H1N1-1918 influenza A viruses.
Gras et al. (2010)
7 more