› 2g5b

X-ray diffraction
2.3Å resolution

Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.

Released:
DOI: 10.2210/pdb2g5b/pdb
PDB entry 2g5b coloured by chain, front view.
PDB entry 2g5b coloured by chain, side view.
PDB entry 2g5b coloured by chain, top view.
Source organisms:
Primary publication:
Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex.
Peyerl FW, Dai S, Murphy GA, Crawford F, White J, Marrack P, Kappler JW
Cell Death Differ 14 447-52 (2007)
PMID: 16946732

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211532 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
6A7 Fab Light Chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 217 amino acids
Theoretical weight: 24.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Ig heavy chain Mem5 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 222 amino acids
Theoretical weight: 23.93 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P84751 (Residues: 1-107, 108-219; Coverage: 92%)
Sequence domains:
Structure domains: Immunoglobulins
Apoptosis regulator BAX Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 7 amino acids
Theoretical weight: 761 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q07812 (Residues: 13-19; Coverage: 4%)
Gene names: BAX, BCL2L4

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21212
Unit cell:
a: 166.528Å b: 183.897Å c: 67.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.275
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

8 review citations

The BCL-2 protein family: opposing activities that mediate cell death.
Youle et al. (2008)
7 more

8 mentions without citation

Toward high-resolution homology modeling of antibody Fv regions and application to antibody-antigen docking.
Sivasubramanian et al. (2009)
7 more