› 2g78

X-ray diffraction
1.7Å resolution

Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinoic Acid at 1.70 Angstroms Resolution

Released:
DOI: 10.2210/pdb2g78/pdb
PDB entry 2g78 coloured by chain, front view.
PDB entry 2g78 coloured by chain, side view.
PDB entry 2g78 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic.
Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B
J Am Chem Soc 129 6140-8 (2007)
PMID: 17447762

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand REA 1 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P212121
Unit cell:
a: 44.702Å b: 46.251Å c: 77.553Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.145 0.203
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The photochemical determinants of color vision: revealing how opsins tune their chromophore's absorption wavelength.
Wang et al. (2014)
26 other articles

2 mentions without citation

QM/MM refinement and analysis of protein bound retinoic acid.
Li et al. (2012)
1 more