› 2g99

X-ray diffraction
1.9Å resolution

Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5

Released:
DOI: 10.2210/pdb2g99/pdb
PDB entry 2g99 coloured by chain, front view.
PDB entry 2g99 coloured by chain, side view.
PDB entry 2g99 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Chai J, Han Z, Wang H, Shen Y

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158476 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 33.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 27-334; Coverage: 92%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 4 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 134.19Å b: 46.89Å c: 112.31Å
α: 90° β: 117.4° γ: 90°
R-values:
R R work R free
0.212 0.212 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

27 review citations

How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
Taverna et al. (2007)
26 more

2 mentions without citation

Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex.
Dias et al. (2014)
1 more