› 2g9n

X-ray diffraction
2.25Å resolution

Structure of the DEAD domain of Human eukaryotic initiation factor 4A, eIF4A

Released:
DOI: 10.2210/pdb2g9n/pdb
PDB entry 2g9n coloured by chain, front view.
PDB entry 2g9n coloured by chain, side view.
PDB entry 2g9n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative structural analysis of human DEAD-box RNA helicases.
Schütz P, Karlberg T, van den Berg S, Collins R, Lehtiö L, Högbom M, Holmberg-Schiavone L, Tempel W, Park HW, Hammarström M, Moche M, Thorsell AG, Schüler H
PLoS One 5 (2010)
PMID: 20941364

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic initiation factor 4A-I Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60842 (Residues: 20-238; Coverage: 54%)
Gene names: DDX2A, EIF4A, EIF4A1
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 28 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 47.799Å b: 78.252Å c: 59.088Å
α: 90° β: 103.43° γ: 90°
R-values:
R R work R free
0.18 0.176 0.257
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Dbp5, Gle1-IP6 and Nup159: a working model for mRNP export.
Folkmann et al. (2011)
16 more

4 mentions without citation

Topology and regulation of the human eIF4A/4G/4H helicase complex in translation initiation.
Marintchev et al. (2009)
3 more