› 2gcn

X-ray diffraction
1.85Å resolution

Crystal structure of the human RhoC-GDP complex

Released:
DOI: 10.2210/pdb2gcn/pdb
PDB entry 2gcn coloured by chain, front view.
PDB entry 2gcn coloured by chain, side view.
PDB entry 2gcn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystal structures reveal two activated states for RhoC.
Dias SM, Cerione RA
Biochemistry 46 6547-58 (2007)
PMID: 17497936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-related GTP-binding protein RhoC Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08134 (Residues: 1-181; Coverage: 94%)
Gene names: ARH9, ARHC, RHOC
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 44.214Å b: 56.617Å c: 81.834Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.225
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Redox control of GTPases: from molecular mechanisms to functional significance in health and disease.
Heo J. (2011)
1 more

2 mentions without citation

Toward understanding RhoGTPase specificity: structure, function and local activation.
Schaefer et al. (2014)
1 more