2gco

X-ray diffraction
1.4Å resolution

Crystal structure of the human RhoC-GppNHp complex

Released:
Source organism: Homo sapiens
Primary publication:
X-ray crystal structures reveal two activated states for RhoC.
Biochemistry 46 6547-58 (2007)
PMID: 17497936

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-139924 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho-related GTP-binding protein RhoC Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08134 (Residues: 1-181; Coverage: 94%)
Gene names: ARH9, ARHC, RHOC
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rho-related GTP-binding protein RhoC Chain: B
Molecule details ›
Chain: B
Length: 201 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08134 (Residues: 1-181; Coverage: 94%)
Gene names: ARH9, ARHC, RHOC
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 64.092Å b: 75.645Å c: 82.903Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.219
Expression system: Escherichia coli