› 2gd5

X-ray diffraction
2.8Å resolution

Structural basis for budding by the ESCRTIII factor CHMP3

Released:
DOI: 10.2210/pdb2gd5/pdb
PDB entry 2gd5 coloured by chain, front view.
PDB entry 2gd5 coloured by chain, side view.
PDB entry 2gd5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for budding by the ESCRT-III factor CHMP3.
Muzioł T, Pineda-Molina E, Ravelli RB, Zamborlini A, Usami Y, Göttlinger H, Weissenhorn W
Dev Cell 10 821-30 (2006)
PMID: 16740483

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Charged multivesicular body protein 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 179 amino acids
Theoretical weight: 21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3E7 (Residues: 9-183; Coverage: 79%)
Gene names: CGI-149, CGI149, CHMP3, NEDF, VPS24
Sequence domains: Snf7
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 52 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 33.58Å b: 72.57Å c: 89.23Å
α: 68.68° β: 83.61° γ: 76.7°
R-values:
R R work R free
0.261 0.259 0.301
Expression system: Escherichia coli

Quick links

Citations

44 review citations

The ESCRT pathway.
Henne et al. (2011)
43 more

11 mentions without citation

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
10 more