› 2geq

X-ray diffraction
2.3Å resolution

Crystal Structure of a p53 Core Dimer Bound to DNA

Released:
DOI: 10.2210/pdb2geq/pdb
PDB entry 2geq coloured by chain, front view.
PDB entry 2geq coloured by chain, side view.
PDB entry 2geq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the p53 core domain dimer bound to DNA.
Ho WC, Fitzgerald MX, Marmorstein R
J Biol Chem 281 20494-502 (2006)
PMID: 16717092

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115295 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 22.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02340 (Residues: 92-292; Coverage: 52%)
Gene names: P53, Tp53, Trp53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
5'-D(*GP*CP*GP*TP*GP*AP*GP*CP*AP*TP*GP*CP*TP*CP*AP*C)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 44.755Å b: 94.29Å c: 119.822Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.238
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Blinded by the Light: The Growing Complexity of p53.
Vousden et al. (2009)
16 more

10 mentions without citation

Towards inferring time dimensionality in protein-protein interaction networks by integrating structures: the p53 example.
Tuncbag et al. (2009)
9 more