› 2gk6

X-ray diffraction
2.4Å resolution

Structural and Functional insights into the human Upf1 helicase core

Released:
DOI: 10.2210/pdb2gk6/pdb
PDB entry 2gk6 coloured by chain, front view.
PDB entry 2gk6 coloured by chain, side view.
PDB entry 2gk6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional insights into the human Upf1 helicase core.
Cheng Z, Muhlrad D, Lim MK, Parker R, Song H
EMBO J 26 253-64 (2007)
PMID: 17159905

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nonsense transcripts 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 624 amino acids
Theoretical weight: 70.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92900 (Residues: 295-925; Coverage: 55%)
Gene names: KIAA0221, RENT1, UPF1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand PO4 2 x PO4
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 63.284Å b: 67.836Å c: 87.25Å
α: 114.41° β: 90.13° γ: 110.22°
R-values:
R R work R free
0.237 0.235 0.272
Expression system: Escherichia coli

Quick links

Citations

32 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
31 more

8 mentions without citation

Structural and functional insights into the human Upf1 helicase core.
Cheng et al. (2007)
7 more