› 2gvg

X-ray diffraction
2.2Å resolution

Crystal Structure of human NMPRTase and its complex with NMN

Released:
DOI: 10.2210/pdb2gvg/pdb
PDB entry 2gvg coloured by chain, front view.
PDB entry 2gvg coloured by chain, side view.
PDB entry 2gvg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the inhibition of human NMPRTase, a novel target for anticancer agents.
Khan JA, Tao X, Tong L
Nat Struct Mol Biol 13 582-8 (2006)
PMID: 16783377

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 491 amino acids
Theoretical weight: 55.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43490 (Residues: 1-491; Coverage: 100%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand PO4 12 x PO4
Ligand NMN 6 x NMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 253.07Å b: 101.367Å c: 148.2Å
α: 90° β: 125.48° γ: 90°
R-values:
R R work R free
0.197 0.197 0.246
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Physiological and pathophysiological roles of NAMPT and NAD metabolism.
Garten et al. (2015)
33 more

5 mentions without citation

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation.
Managò et al. (2019)
4 more