› 2h2b

X-ray diffraction
1.6Å resolution

Crystal Structure of ZO-1 PDZ1 Bound to a Phage-Derived Ligand (WRRTTYL)

Released:
DOI: 10.2210/pdb2h2b/pdb
PDB entry 2h2b coloured by chain, front view.
PDB entry 2h2b coloured by chain, side view.
PDB entry 2h2b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative structural analysis of the Erbin PDZ domain and the first PDZ domain of ZO-1. Insights into determinants of PDZ domain specificity.
Appleton BA, Zhang Y, Wu P, Yin JP, Hunziker W, Skelton NJ, Sidhu SS, Wiesmann C
J Biol Chem 281 22312-22320 (2006)
PMID: 16737969

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tight junction protein ZO-1 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07157 (Residues: 18-110; Coverage: 5%)
Gene names: TJP1, ZO1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 52.425Å b: 52.425Å c: 92.932Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Engineering of recombinant crystallization chaperones.
Koide S. (2009)
9 more

7 mentions without citation

A specificity map for the PDZ domain family.
Tonikian et al. (2008)
6 more