› 2h2n

X-ray diffraction
2.3Å resolution

Crystal structure of human soluble calcium-activated nucleotidase (SCAN) with calcium ion

Released:
DOI: 10.2210/pdb2h2n/pdb
PDB entry 2h2n coloured by chain, front view.
PDB entry 2h2n coloured by chain, side view.
PDB entry 2h2n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Calcium-dependent dimerization of human soluble calcium activated nucleotidase: characterization of the dimer interface.
Yang M, Horii K, Herr AB, Kirley TL
J Biol Chem 281 28307-17 (2006)
PMID: 16835225

Function and Biology Details

Reaction catalysed: 
A nucleoside diphosphate + H(2)O = a nucleoside phosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble calcium-activated nucleotidase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 37.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WVQ1 (Residues: 69-401; Coverage: 83%)
Gene names: CANT1, SHAPY
Sequence domains: Apyrase
Structure domains: Apyrase

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 43.094Å b: 52.368Å c: 77.426Å
α: 75.06° β: 74.52° γ: 79.47°
R-values:
R R work R free
0.172 0.168 0.249
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

The first crystal structure of gluconolactonase important in the glucose secondary metabolic pathways.
Chen et al. (2008)
5 more

4 mentions without citation

Micellar structure in gemini nonionic surfactants from small-angle neutron scattering.
Fitzgerald et al. (2005)
3 more