› 2h3n

X-ray diffraction
2.3Å resolution

Crystal structure of a surrogate light chain (LAMBDA5 and VpreB) homodimer

Released:
DOI: 10.2210/pdb2h3n/pdb
PDB entry 2h3n coloured by chain, front view.
PDB entry 2h3n coloured by chain, side view.
PDB entry 2h3n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into pre-B cell receptor function.
Bankovich AJ, Raunser S, Juo ZS, Walz T, Davis MM, Garcia KC
Science 316 291-4 (2007)
PMID: 17431183

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146073 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin iota chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 100 amino acids
Theoretical weight: 11.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P12018 (Residues: 21-119; Coverage: 79%)
Gene names: VPREB, VPREB1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Immunoglobulin lambda-like polypeptide 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 117 amino acids
Theoretical weight: 12.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P15814 (Residues: 94-209; Coverage: 66%)
Gene names: IGL1, IGLL1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 60.169Å b: 72.814Å c: 115.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.281
Expression system: Trichoplusia ni

Quick links

Citations

24 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
23 more

5 mentions without citation

A complete, multi-level conformational clustering of antibody complementarity-determining regions.
Nikoloudis et al. (2014)
4 more