2h44

X-ray diffraction
1.8Å resolution

Crystal structure of PDE5A1 in complex with icarisid II

Released:

Function and Biology Details

Reaction catalysed:
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-131208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-specific 3',5'-cyclic phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 37.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O76074 (Residues: 535-860; Coverage: 37%)
Gene names: PDE5, PDE5A
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 110.746Å b: 110.746Å c: 106.159Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.233
Expression system: Escherichia coli BL21