› 2h88

X-ray diffraction
1.74Å resolution

Avian Mitochondrial Respiratory Complex II at 1.8 Angstrom Resolution

Released:
DOI: 10.2210/pdb2h88/pdb
PDB entry 2h88 coloured by chain, front view.
PDB entry 2h88 coloured by chain, side view.
PDB entry 2h88 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystallographic studies of the binding of ligands to the dicarboxylate site of Complex II, and the identity of the ligand in the "oxaloacetate-inhibited" state.
Huang LS, Shen JT, Wang AC, Berry EA
Biochim Biophys Acta 1757 1073-83
PMID: 16935256

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero heptamer
PDBe Complex ID:
PDB-CPX-112133 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Chains: A, N
Molecule details ›
Chains: A, N
Length: 621 amino acids
Theoretical weight: 68.26 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: Q9YHT1 (Residues: 45-665; Coverage: 93%)
Gene name: SDHA
Sequence domains:
Structure domains:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Chains: B, O
Molecule details ›
Chains: B, O
Length: 252 amino acids
Theoretical weight: 28.69 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: Q9YHT2 (Residues: 39-290; Coverage: 87%)
Gene name: SDHB
Sequence domains:
Structure domains:
Succinate dehydrogenase cytochrome b560 subunit, mitochondrial Chains: C, P
Molecule details ›
Chains: C, P
Length: 140 amino acids
Theoretical weight: 15.39 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: D0VWW3 (Residues: 1-140; Coverage: 100%)
Sequence domains: Succinate dehydrogenase/Fumarate reductase transmembrane subunit
Structure domains:
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 103 amino acids
Theoretical weight: 10.97 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: Q5ZIS0 (Residues: 55-157; Coverage: 66%)
Gene names: RCJMB04_23p7, SDHD
Sequence domains: CybS, succinate dehydrogenase cytochrome B small subunit
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM

Cofactor: Ligand FAD 2 x FAD
9 bound ligands:
Ligand K 4 x K
Ligand AZI 2 x AZI
Ligand TEO 2 x TEO
Ligand UNL 64 x UNL
Ligand FES 2 x FES
Ligand SF4 2 x SF4
Ligand F3S 2 x F3S
Ligand GOL 2 x GOL
Ligand BHG 2 x BHG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 119.972Å b: 199.391Å c: 68.063Å
α: 90° β: 90.35° γ: 90°
R-values:
R R work R free
0.179 0.178 0.206

Quick links

Citations

6 review citations

The mitochondrial protein import machinery has multiple connections to the respiratory chain.
Kulawiak et al. (2013)
5 more

17 mentions without citation

Review: studies of ferric heme proteins with highly anisotropic/highly axial low spin (S = 1/2) electron paramagnetic resonance signals with bis-histidine and histidine-methionine axial iron coordination.
Zoppellaro et al. (2009)
16 more