› 2h8l

X-ray diffraction
2Å resolution

Crystal structure of the bb' fragment of ERp57

Released:
DOI: 10.2210/pdb2h8l/pdb
PDB entry 2h8l coloured by chain, front view.
PDB entry 2h8l coloured by chain, side view.
PDB entry 2h8l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the bb' domains of the protein disulfide isomerase ERp57.
Kozlov G, Maattanen P, Schrag JD, Pollock S, Cygler M, Nagar B, Thomas DY, Gehring K
Structure 14 1331-9 (2006)
PMID: 16905107

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein disulfide-isomerase A3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 252 amino acids
Theoretical weight: 28.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30101 (Residues: 134-376; Coverage: 51%)
Gene names: ERP57, ERP60, GRP58, PDIA3
Sequence domains: Thioredoxin-like domain
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 76.34Å b: 62.415Å c: 99.293Å
α: 90° β: 98.4° γ: 90°
R-values:
R R work R free
0.197 0.194 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

32 review citations

In and out of the ER: protein folding, quality control, degradation, and related human diseases.
Hebert et al. (2007)
31 more

9 mentions without citation

Calnexin cycle - structural features of the ER chaperone system.
Kozlov et al. (2020)
8 more