› 2hg5

X-ray diffraction
2.75Å resolution

Cs+ complex of a K channel with an amide to ester substitution in the selectivity filter

Released:
DOI: 10.2210/pdb2hg5/pdb
PDB entry 2hg5 coloured by chain, front view.
PDB entry 2hg5 coloured by chain, side view.
PDB entry 2hg5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel.
Valiyaveetil FI, Sekedat M, MacKinnon R, Muir TW
J Am Chem Soc 128 11591-9 (2006)
PMID: 16939283

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-242257 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q569B4 (Residues: 20-78, 79-243; Coverage: 49%)
Gene name: Ighg
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-105; Coverage: 98%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 101 amino acids
Theoretical weight: 10.78 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P0A334 (Residues: 22-122; Coverage: 63%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand CS 4 x CS
Ligand B3H 1 x B3H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4
Unit cell:
a: 156.597Å b: 156.597Å c: 75.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.271
Expression system: Not provided

Quick links

Citations

13 review citations

An evaluation of peptide-bond isosteres.
Choudhary et al. (2011)
12 more

1 mention without citation

Scalable Production of Graphene-Based Wearable E-Textiles.
Karim et al. (2017)