› 2hp4

X-ray diffraction
2.1Å resolution

Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor

Released:
DOI: 10.2210/pdb2hp4/pdb
PDB entry 2hp4 coloured by chain, front view.
PDB entry 2hp4 coloured by chain, side view.
PDB entry 2hp4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor.
Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF
Proteins 67 65-74 (2007)
PMID: 17243170

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
T-cell surface glycoprotein CD8 alpha chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01732 (Residues: 22-135; Coverage: 53%)
Gene names: CD8A, MAL
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P64
Unit cell:
a: 99.723Å b: 99.723Å c: 55.806Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.233
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Survey of the year 2007 commercial optical biosensor literature.
Rich et al. (2008)
2 more

2 mentions without citation

The CD8α hinge is intrinsically disordered with a dynamic exchange that includes proline cis-trans isomerization.
Chen et al. (2022)
1 more