› 2hw1

X-ray diffraction
2.1Å resolution

Crystal structure of human ketohexokinase complexed to different sugar molecules

Released:
DOI: 10.2210/pdb2hw1/pdb
PDB entry 2hw1 coloured by chain, front view.
PDB entry 2hw1 coloured by chain, side view.
PDB entry 2hw1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of alternatively spliced isoforms of human ketohexokinase.
Trinh CH, Asipu A, Bonthron DT, Phillips SE
Acta Crystallogr D Biol Crystallogr 65 201-11 (2009)
PMID: 19237742

Function and Biology Details

Reaction catalysed: 
ATP + D-fructose = ADP + D-fructose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketohexokinase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 32.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50053 (Residues: 1-298; Coverage: 100%)
Gene name: KHK
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

4 bound ligands:
Ligand FRU 1 x FRU
Ligand SO4 1 x SO4
Ligand ANP 1 x ANP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: I222
Unit cell:
a: 61.264Å b: 107.823Å c: 146.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.228
Expression system: Escherichia coli

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
12 other articles

6 mentions without citation

Ketohexokinase: expression and localization of the principal fructose-metabolizing enzyme.
Diggle et al. (2009)
5 more