› 2hww

X-ray diffraction
1.8Å resolution

Structure of PIN domain of human SMG6

Released:
DOI: 10.2210/pdb2hww/pdb
PDB entry 2hww coloured by chain, front view.
PDB entry 2hww coloured by chain, side view.
PDB entry 2hww coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex.
Glavan F, Behm-Ansmant I, Izaurralde E, Conti E
EMBO J 25 5117-25 (2006)
PMID: 17053788

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Telomerase-binding protein EST1A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 181 amino acids
Theoretical weight: 20.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q86US8 (Residues: 1239-1419; Coverage: 13%)
Gene names: C17orf31, EST1A, KIAA0732, SMG6
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 36.879Å b: 71.216Å c: 181.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.267
Expression system: Escherichia coli BL21

Quick links

Citations

34 review citations

P bodies: at the crossroads of post-transcriptional pathways.
Eulalio et al. (2007)
33 more

11 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
10 more