› 2hwy

X-ray diffraction
2.75Å resolution

Structure of PIN domain of human SMG5.

Released:
DOI: 10.2210/pdb2hwy/pdb
PDB entry 2hwy coloured by chain, front view.
PDB entry 2hwy coloured by chain, side view.
PDB entry 2hwy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex.
Glavan F, Behm-Ansmant I, Izaurralde E, Conti E
EMBO J 25 5117-25 (2006)
PMID: 17053788

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nonsense-mediated mRNA decay factor SMG5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UPR3 (Residues: 853-1016; Coverage: 16%)
Gene names: EST1B, KIAA1089, SMG5
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 45.591Å b: 42.534Å c: 65.103Å
α: 90° β: 95.98° γ: 90°
R-values:
R R work R free
0.271 0.271 0.329
Expression system: Escherichia coli BL21

Quick links

Citations

34 review citations

P bodies: at the crossroads of post-transcriptional pathways.
Eulalio et al. (2007)
33 more

5 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
4 more