› 2hxk

X-ray diffraction
1.65Å resolution

Crystal structure of S-nitroso thioredoxin

Released:
DOI: 10.2210/pdb2hxk/pdb
PDB entry 2hxk coloured by chain, front view.
PDB entry 2hxk coloured by chain, side view.
PDB entry 2hxk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Buried S-nitrosocysteine revealed in crystal structures of human thioredoxin.
Weichsel A, Brailey JL, Montfort WR
Biochemistry 46 1219-27 (2007)
PMID: 17260951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 105 amino acids
Theoretical weight: 11.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10599 (Residues: 1-105; Coverage: 100%)
Gene names: TRDX, TRX, TRX1, TXN
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand EOH 3 x EOH
1 modified residue:
Ligand SNC 6 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 116.35Å b: 25.74Å c: 86.02Å
α: 90° β: 98.2° γ: 90°
R-values:
R R work R free
0.197 0.195 0.239
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Protein denitrosylation: enzymatic mechanisms and cellular functions.
Benhar et al. (2009)
9 more

4 mentions without citation

Redox biology: computational approaches to the investigation of functional cysteine residues.
Marino et al. (2011)
3 more