› 2hxm

X-ray diffraction
1.3Å resolution

Complex of UNG2 and a small Molecule synthetic Inhibitor

Released:
DOI: 10.2210/pdb2hxm/pdb
PDB entry 2hxm coloured by chain, front view.
PDB entry 2hxm coloured by chain, side view.
PDB entry 2hxm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mimicking damaged DNA with a small molecule inhibitor of human UNG2.
Krosky DJ, Bianchet MA, Seiple L, Chung S, Amzel LM, Stivers JT
Nucleic Acids Res 34 5872-9 (2006)
PMID: 17062624

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13051 (Residues: 94-313; Coverage: 70%)
Gene names: DGU, UNG, UNG1, UNG15
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand 302 1 x 302
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 43.209Å b: 69.03Å c: 70.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.206
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Uracil-DNA glycosylase: Structural, thermodynamic and kinetic aspects of lesion search and recognition.
Zharkov et al. (2010)
3 more

4 mentions without citation

Inhibitors of DNA Glycosylases as Prospective Drugs.
Mechetin et al. (2020)
3 more