› 2i5f

X-ray diffraction
1.35Å resolution

Crystal structure of the C-terminal PH domain of pleckstrin in complex with D-myo-Ins(1,2,3,5,6)P5

Released:
DOI: 10.2210/pdb2i5f/pdb
PDB entry 2i5f coloured by chain, front view.
PDB entry 2i5f coloured by chain, side view.
PDB entry 2i5f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the carboxy terminal PH domain of pleckstrin bound to D-myo-inositol 1,2,3,5,6-pentakisphosphate.
Jackson SG, Zhang Y, Haslam RJ, Junop MS
BMC Struct Biol 7 80 (2007)
PMID: 18034889

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140179 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pleckstrin Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08567 (Residues: 244-347; Coverage: 30%)
Gene names: P47, PLEK
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand 5IP 1 x 5IP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 32.15Å b: 47.7Å c: 64.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Inositol pyrophosphates: structure, enzymology and function.
Barker et al. (2009)
2 more

13 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
12 more