› 2i9p

X-ray diffraction
2.55Å resolution

Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+

Released:
DOI: 10.2210/pdb2i9p/pdb
PDB entry 2i9p coloured by chain, front view.
PDB entry 2i9p coloured by chain, side view.
PDB entry 2i9p coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kavanagh KL, Papagrigoriou E, Salah E, Lukacik P, Smee C, Burgess N, von Delft F, Weigelt J, Arrowsmith C, Sundstrom M, Edwards A, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyisobutyrate dehydrogenase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 33.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31937 (Residues: 40-336; Coverage: 88%)
Gene name: HIBADH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 49.627Å b: 167.319Å c: 71.089Å
α: 90° β: 107.8° γ: 90°
R-values:
R R work R free
0.176 0.174 0.22
Expression system: Escherichia coli BL21(DE3)

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