› 2ibn

X-ray diffraction
1.5Å resolution

Crystal structure of Human myo-Inositol Oxygenase (MIOX)

Released:
DOI: 10.2210/pdb2ibn/pdb
PDB entry 2ibn coloured by chain, front view.
PDB entry 2ibn coloured by chain, side view.
PDB entry 2ibn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biophysical characterization of human myo-inositol oxygenase.
Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarström M, Gräslund S, Gräslund A, Hallberg BM
J Biol Chem 283 15209-16 (2008)
PMID: 18364358

Function and Biology Details

Reaction catalysed: 
Myo-inositol + O(2) = D-glucuronate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol oxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 29.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UGB7 (Residues: 38-285; Coverage: 87%)
Gene names: ALDRL6, KSP32, MIOX, RSOR
Sequence domains: Myo-inositol oxygenase

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand SO4 2 x SO4
Ligand I1N 2 x I1N
Ligand CYS 2 x CYS
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 119.715Å b: 55.861Å c: 111.516Å
α: 90° β: 116.72° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
5 more

6 mentions without citation

Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases.
Wörsdörfer et al. (2013)
5 more