› 2ic1

X-ray diffraction
2.7Å resolution

Crystal Structure of Human Cysteine Dioxygenase in Complex with Substrate Cysteine

Released:
DOI: 10.2210/pdb2ic1/pdb
PDB entry 2ic1 coloured by chain, front view.
PDB entry 2ic1 coloured by chain, side view.
PDB entry 2ic1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An insight into the mechanism of human cysteine dioxygenase. Key roles of the thioether-bonded tyrosine-cysteine cofactor.
Ye S, Wu X, Wei L, Tang D, Sun P, Bartlam M, Rao Z
J Biol Chem 282 3391-402 (2007)
PMID: 17135237

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16878 (Residues: 1-200; Coverage: 100%)
Gene name: CDO1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand CYS 2 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 131.1Å b: 131.1Å c: 34.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.178 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Enzymology of H2S biogenesis, decay and signaling.
Kabil et al. (2014)
15 more

13 mentions without citation

Structure and function of atypically coordinated enzymatic mononuclear non-heme-Fe(II) centers.
Buongiorno et al. (2013)
12 more