› 2ifd

X-ray diffraction
2Å resolution

Crystal structure of a remote binding site mutant, R492L, of CDC25B Phosphatase catalytic domain

Released:
DOI: 10.2210/pdb2ifd/pdb
PDB entry 2ifd coloured by chain, front view.
PDB entry 2ifd coloured by chain, side view.
PDB entry 2ifd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic and structural studies of specific protein-protein interactions in substrate catalysis by Cdc25B phosphatase.
Sohn J, Buhrman G, Rudolph J
Biochemistry 46 807-18 (2007)
PMID: 17223702

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30305 (Residues: 391-564; Coverage: 30%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 49.957Å b: 71.32Å c: 75.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Survey of the year 2007 commercial optical biosensor literature.
Rich et al. (2008)
3 more