› 2igp

X-ray diffraction
1.8Å resolution

Crystal Structure of Hec1 CH domain

Released:
DOI: 10.2210/pdb2igp/pdb
PDB entry 2igp coloured by chain, front view.
PDB entry 2igp coloured by chain, side view.
PDB entry 2igp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment.
Wei RR, Al-Bassam J, Harrison SC
Nat Struct Mol Biol 14 54-9 (2007)
PMID: 17195848

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinetochore protein NDC80 homolog Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14777 (Residues: 81-196; Coverage: 18%)
Gene names: HEC, HEC1, KNTC2, NDC80
Sequence domains: HEC/Ndc80p family
Structure domains: Ncd80 complex, Ncd80 subunit

Ligands and Environments

1 bound ligand:
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2, ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 40.929Å b: 44.703Å c: 68.036Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.231
Expression system: Escherichia coli

Quick links

Citations

70 review citations

Tracking the ends: a dynamic protein network controls the fate of microtubule tips.
Akhmanova et al. (2008)
69 more

5 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
4 more