› 2ihw

X-ray diffraction
2.7Å resolution

Crystal structure of a cubic core of the dihydrolipoamide acyltransferase (E2b) component in the branched-chain alpha-ketoacid dehydrogenase complex (BCKDC), apo form

Released:
DOI: 10.2210/pdb2ihw/pdb
PDB entry 2ihw coloured by chain, front view.
PDB entry 2ihw coloured by chain, side view.
PDB entry 2ihw coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain alpha-ketoacid dehydrogenase complex.
Kato M, Wynn RM, Chuang JL, Brautigam CA, Custorio M, Chuang DT
EMBO J 25 5983-94 (2006)
PMID: 17124494

Function and Biology Details

Reaction catalysed: 
2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 262 amino acids
Theoretical weight: 28.73 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11181 (Residues: 223-482; Coverage: 54%)
Gene name: DBT
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

2 bound ligands:
Ligand ACT 16 x ACT
Ligand CL 12 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 194.654Å b: 194.654Å c: 171.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.257
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Lipoic acid metabolism and mitochondrial redox regulation.
Solmonson et al. (2018)
2 more

2 mentions without citation

A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain alpha-ketoacid dehydrogenase complex.
Kato et al. (2006)
1 more