› 2ii4

X-ray diffraction
2.59Å resolution

Crystal structure of a cubic core of the dihydrolipoamide acyltransferase (E2b) component in the branched-chain alpha-ketoacid dehydrogenase complex (BCKDC), Coenzyme A-bound form

Released:
DOI: 10.2210/pdb2ii4/pdb
PDB entry 2ii4 coloured by chain, front view.
PDB entry 2ii4 coloured by chain, side view.
PDB entry 2ii4 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain alpha-ketoacid dehydrogenase complex.
Kato M, Wynn RM, Chuang JL, Brautigam CA, Custorio M, Chuang DT
EMBO J 25 5983-94 (2006)
PMID: 17124494

Function and Biology Details

Reaction catalysed: 
2-methylpropanoyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-(2-methylpropanoyl)dihydrolipoyl)lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 262 amino acids
Theoretical weight: 28.73 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11181 (Residues: 223-482; Coverage: 54%)
Gene name: DBT
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments


Cofactor: Ligand COA 8 x COA
1 bound ligand:
Ligand CL 12 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 195.234Å b: 195.234Å c: 172.756Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Lipoic acid metabolism and mitochondrial redox regulation.
Solmonson et al. (2018)
2 more

4 mentions without citation

Structure and function of the catalytic domain of the dihydrolipoyl acetyltransferase component in Escherichia coli pyruvate dehydrogenase complex.
Wang et al. (2014)
3 more