› 2iiy

X-ray diffraction
1.7Å resolution

Crystal structure of S-nitroso thioredoxin

Released:
DOI: 10.2210/pdb2iiy/pdb
PDB entry 2iiy coloured by chain, front view.
PDB entry 2iiy coloured by chain, side view.
PDB entry 2iiy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Buried S-nitrosocysteine revealed in crystal structures of human thioredoxin.
Weichsel A, Brailey JL, Montfort WR
Biochemistry 46 1219-27 (2007)
PMID: 17260951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10599 (Residues: 1-105; Coverage: 100%)
Gene names: TRDX, TRX, TRX1, TXN
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SNC 2 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 66.86Å b: 26.24Å c: 51.3Å
α: 90° β: 95.02° γ: 90°
R-values:
R R work R free
0.21 0.209 0.239
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Protein denitrosylation: enzymatic mechanisms and cellular functions.
Benhar et al. (2009)
10 more

2 mentions without citation

First report of a thioredoxin homologue in jellyfish: molecular cloning, expression and antioxidant activity of CcTrx1 from Cyanea capillata.
Ruan et al. (2014)
1 more