› 2isi

X-ray diffraction
2.76Å resolution

Crystal structure of Ape1 from Homo sapiens in a new crystal form complexed with a ligand

Released:
DOI: 10.2210/pdb2isi/pdb
PDB entry 2isi coloured by chain, front view.
PDB entry 2isi coloured by chain, side view.
PDB entry 2isi coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Agarwal R, Naidu MD

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair nuclease/redox regulator APEX1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 317 amino acids
Theoretical weight: 35.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27695 (Residues: 2-318; Coverage: 100%)
Gene names: APE, APE1, APEX, APEX1, APX, HAP1, REF1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 167.347Å b: 92.392Å c: 94.006Å
α: 90° β: 121.38° γ: 90°
R-values:
R R work R free
0.24 0.228 0.284
Expression system: Escherichia coli BL21(DE3)

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