› 2it5

X-ray diffraction
2.4Å resolution

Crystal Structure of DCSIGN-CRD with man6

Released:
DOI: 10.2210/pdb2it5/pdb
PDB entry 2it5 coloured by chain, front view.
PDB entry 2it5 coloured by chain, side view.
PDB entry 2it5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple modes of binding enhance the affinity of DC-SIGN for high mannose N-linked glycans found on viral glycoproteins.
Feinberg H, Castelli R, Drickamer K, Seeberger PH, Weis WI
J Biol Chem 282 4202-9 (2007)
PMID: 17150970

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CD209 antigen Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 16.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NNX6 (Residues: 250-388; Coverage: 34%)
Gene names: CD209, CLEC4L
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43
Unit cell:
a: 55.96Å b: 55.96Å c: 53.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Protein-glycan interactions in the control of innate and adaptive immune responses.
van Kooyk et al. (2008)
17 more

11 mentions without citation

Molecular Recognition in C-Type Lectins: The Cases of DC-SIGN, Langerin, MGL, and L-Sectin.
Valverde et al. (2020)
10 more