› 2ivs

X-ray diffraction
2Å resolution

Crystal structure of non-phosphorylated RET tyrosine kinase domain

Released:
DOI: 10.2210/pdb2ivs/pdb
PDB entry 2ivs coloured by chain, front view.
PDB entry 2ivs coloured by chain, side view.
PDB entry 2ivs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and chemical inhibition of the RET tyrosine kinase domain.
Knowles PP, Murray-Rust J, Kjaer S, Scott RP, Hanrahan S, Santoro M, Ibáñez CF, McDonald NQ
J Biol Chem 281 33577-87 (2006)
PMID: 16928683

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble RET kinase fragment Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07949 (Residues: 703-1013; Coverage: 29%)
Gene names: CDHF12, CDHR16, PTC, RET
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 6 x FMT
Ligand ACK 2 x ACK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 50.399Å b: 80.217Å c: 79.684Å
α: 90° β: 100.09° γ: 90°
R-values:
R R work R free
0.183 0.18 0.229
Expression system: Spodoptera frugiperda

Quick links

Citations

66 review citations

Cell signaling by receptor tyrosine kinases.
Lemmon et al. (2010)
65 more

9 mentions without citation

Forcefield_PTM: Ab Initio Charge and AMBER Forcefield Parameters for Frequently Occurring Post-Translational Modifications.
Khoury et al. (2013)
8 more