› 2iwg

X-ray diffraction
2.35Å resolution

COMPLEX BETWEEN THE PRYSPRY DOMAIN OF TRIM21 AND IGG FC

Released:
DOI: 10.2210/pdb2iwg/pdb
PDB entry 2iwg coloured by chain, front view.
PDB entry 2iwg coloured by chain, side view.
PDB entry 2iwg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function.
James LC, Keeble AH, Khan Z, Rhodes DA, Trowsdale J
Proc Natl Acad Sci U S A 104 6200-5 (2007)
PMID: 17400754

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134857 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 207 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01857 (Residues: 120-326; Coverage: 52%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
E3 ubiquitin-protein ligase TRIM21 Chains: B, E
Molecule details ›
Chains: B, E
Length: 181 amino acids
Theoretical weight: 20.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19474 (Residues: 287-465; Coverage: 38%)
Gene names: RNF81, RO52, SSA1, TRIM21
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
1 bound ligand:
Ligand FUC 2 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 112.49Å b: 112.49Å c: 194.609Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.253
Expression system: Escherichia coli

Quick links

Citations

64 review citations

TRIM family proteins and their emerging roles in innate immunity.
Ozato et al. (2008)
63 more

28 mentions without citation

Structure and function of the SPRY/B30.2 domain proteins involved in innate immunity.
D'Cruz et al. (2013)
27 more