› 2j1d

X-ray diffraction
2.25Å resolution

Crystallization of hDaam1 C-terminal Fragment

Released:
DOI: 10.2210/pdb2j1d/pdb
PDB entry 2j1d coloured by chain, front view.
PDB entry 2j1d coloured by chain, side view.
PDB entry 2j1d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.
Lu J, Meng W, Poy F, Maiti S, Goode BL, Eck MJ
J Mol Biol 369 1258-69 (2007)
PMID: 17482208

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disheveled-associated activator of morphogenesis 1 Chain: G
Molecule details ›
Chain: G
Length: 483 amino acids
Theoretical weight: 56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y4D1 (Residues: 596-1078; Coverage: 45%)
Gene names: DAAM1, KIAA0666
Sequence domains: Formin Homology 2 Domain
Structure domains: Formin, FH2 domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 100.897Å b: 100.343Å c: 148.397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

A nucleator arms race: cellular control of actin assembly.
Campellone et al. (2010)
11 more

9 mentions without citation

Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.
Lu et al. (2007)
8 more