› 2j3s

X-ray diffraction
2.5Å resolution

Crystal structure of the human filamin A Ig domains 19 to 21

Released:
DOI: 10.2210/pdb2j3s/pdb
PDB entry 2j3s coloured by chain, front view.
PDB entry 2j3s coloured by chain, side view.
PDB entry 2j3s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of three tandem filamin domains reveals auto-inhibition of ligand binding.
Lad Y, Kiema T, Jiang P, Pentikäinen OT, Coles CH, Campbell ID, Calderwood DA, Ylänne J
EMBO J 26 3993-4004 (2007)
PMID: 17690686

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamin-A Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 30.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21333 (Residues: 2045-2329; Coverage: 11%)
Gene names: FLN, FLN1, FLNA
Sequence domains: Filamin/ABP280 repeat
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand BR 2 x BR
Ligand DIO 4 x DIO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 72.28Å b: 78.39Å c: 229.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.252 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Filamins in mechanosensing and signaling.
Razinia et al. (2012)
16 more

19 mentions without citation

The filamins: organizers of cell structure and function.
Nakamura et al. (2011)
18 more