› 2j3t

X-ray diffraction
2.4Å resolution

The crystal structure of the bet3-trs33-bet5-trs23 complex.

Released:
DOI: 10.2210/pdb2j3t/pdb
PDB entry 2j3t coloured by chain, front view.
PDB entry 2j3t coloured by chain, side view.
PDB entry 2j3t coloured by chain, top view.
Source organisms:
Primary publication:
The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering.
Kim YG, Raunser S, Munger C, Wagner J, Song YL, Cygler M, Walz T, Oh BH, Sacher M
Cell 127 817-30 (2006)
PMID: 17110339

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129535 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Trafficking protein particle complex subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O55013 (Residues: 1-180; Coverage: 100%)
Gene names: Bet3, Trappc3
Sequence domains: Transport protein particle (TRAPP) component
Structure domains: Trafficking protein particle complex subunit 3
Trafficking protein particle complex subunit 6A Chain: B
Molecule details ›
Chain: B
Length: 159 amino acids
Theoretical weight: 17.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75865 (Residues: 1-159; Coverage: 100%)
Gene names: HSPC289, TRAPPC6A
Sequence domains: Transport protein particle (TRAPP) component
Structure domains: Trafficking protein particle complex subunit 3
Trafficking protein particle complex subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 145 amino acids
Theoretical weight: 16.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NCF2 (Residues: 1-145; Coverage: 100%)
Gene name: Trappc1
Sequence domains: Sybindin-like family
Structure domains: Beta-Lactamase
Trafficking protein particle complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 219 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y296 (Residues: 1-219; Coverage: 100%)
Gene names: CGI-104, HSPC172, PTD009, SBDN, TRAPPC4
Sequence domains: Sybindin-like family
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 57.602Å b: 66.336Å c: 200.808Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Role of Rab GTPases in membrane traffic and cell physiology.
Hutagalung et al. (2011)
39 more

11 mentions without citation

Next-generation electron microscopy in autophagy research.
Hurley et al. (2016)
10 more