› 2j4y

X-ray diffraction
3.4Å resolution

Crystal structure of a rhodopsin stabilizing mutant expressed in mammalian cells

Released:
DOI: 10.2210/pdb2j4y/pdb
PDB entry 2j4y coloured by chain, front view.
PDB entry 2j4y coloured by chain, side view.
PDB entry 2j4y coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of a thermally stable rhodopsin mutant.
Standfuss J, Xie G, Edwards PC, Burghammer M, Oprian DD, Schertler GF
J Mol Biol 372 1179-88 (2007)
PMID: 17825322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
Expression system: Chlorocebus aethiops
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P31
Unit cell:
a: 109.3Å b: 109.3Å c: 77.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.29 0.29 0.33
Expression system: Chlorocebus aethiops

Quick links

Citations

42 review citations

The structure and function of G-protein-coupled receptors.
Rosenbaum et al. (2009)
41 more

32 mentions without citation

Diversity and modularity of G protein-coupled receptor structures.
Katritch et al. (2012)
31 more