› 2j6e

X-ray diffraction
3Å resolution

Crystal Structure of an Autoimmune Complex between a Human IgM Rheumatoid Factor and IgG1 Fc reveals a Novel Fc Epitope and Evidence for Affinity Maturation

Released:
DOI: 10.2210/pdb2j6e/pdb
PDB entry 2j6e coloured by chain, front view.
PDB entry 2j6e coloured by chain, side view.
PDB entry 2j6e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a human autoimmune complex between IgM rheumatoid factor RF61 and IgG1 Fc reveals a novel epitope and evidence for affinity maturation.
Duquerroy S, Stura EA, Bressanelli S, Fabiane SM, Vaney MC, Beale D, Hamon M, Casali P, Rey FA, Sutton BJ, Taussig MJ
J Mol Biol 368 1321-31 (2007)
PMID: 17395205

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208631 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 26.14 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 99-328; Coverage: 58%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
IGM Chains: H, I
Molecule details ›
Chains: H, I
Length: 231 amino acids
Theoretical weight: 24.83 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
IGM Chains: L, M
Molecule details ›
Chains: L, M
Length: 234 amino acids
Theoretical weight: 24.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUL
Carbohydrate polymer
5 bound ligands:
Ligand CD 1 x CD
Ligand ZN 2 x ZN
Ligand CAC 1 x CAC
Ligand MPD 6 x MPD
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 241.98Å b: 75.61Å c: 102.4Å
α: 90° β: 91.13° γ: 90°
R-values:
R R work R free
0.223 0.223 0.288
Expression systems:
  • Cricetulus griseus
  • Homo sapiens

Quick links

Citations

9 review citations

Nature and functions of autoantibodies.
Elkon et al. (2008)
8 more

9 mentions without citation

Structural analysis of B-cell epitopes in antibody:protein complexes.
Kringelum et al. (2013)
8 more