› 2j8z

X-ray diffraction
2.5Å resolution

Crystal Structure of human P53 inducible oxidoreductase (TP53I3,PIG3)

Released:
DOI: 10.2210/pdb2j8z/pdb
PDB entry 2j8z coloured by chain, front view.
PDB entry 2j8z coloured by chain, side view.
PDB entry 2j8z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Three-dimensional structure and enzymatic function of proapoptotic human p53-inducible quinone oxidoreductase PIG3.
Porté S, Valencia E, Yakovtseva EA, Borràs E, Shafqat N, Debreczeny JÉ, Pike ACW, Oppermann U, Farrés J, Fita I, Parés X
J Biol Chem 284 17194-17205 (2009)
PMID: 19349281

Function and Biology Details

Reaction catalysed: 
NADPH + 2 quinone = NADP(+) + 2 semiquinone
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinone oxidoreductase PIG3 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 38.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53FA7 (Residues: 1-332; Coverage: 100%)
Gene names: PIG3, TP53I3
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P4322
Unit cell:
a: 46.86Å b: 46.86Å c: 315.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.271
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Oxidative Stress in Cancer.
Hayes et al. (2020)
6 more

7 mentions without citation

Divergence of multimodular polyketide synthases revealed by a didomain structure.
Zheng et al. (2012)
6 more