› 2j9h

X-ray diffraction
2.4Å resolution

Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution

Released:
DOI: 10.2210/pdb2j9h/pdb
PDB entry 2j9h coloured by chain, front view.
PDB entry 2j9h coloured by chain, side view.
PDB entry 2j9h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modulating catalytic activity by unnatural amino acid residues in a GSH-binding loop of GST P1-1.
Hegazy UM, Tars K, Hellman U, Mannervik B
J Mol Biol 376 811-26 (2008)
PMID: 18177897

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase P Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09211 (Residues: 2-210; Coverage: 100%)
Gene names: FAEES3, GST3, GSTP1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTX 2 x GTX
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P43212
Unit cell:
a: 59.566Å b: 59.566Å c: 238.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.25 0.31
Expression system: Escherichia coli

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Citations

6 citation in other articles

Comprehensive profiling of metastasis-related proteins in paired hepatocellular carcinoma cells with different metastasis potentials.
Song et al. (2009)
5 more

1 mention without citation

PFresGO: an attention mechanism-based deep-learning approach for protein annotation by integrating gene ontology inter-relationships.
Pan et al. (2023)