› 2jb5

X-ray diffraction
2.8Å resolution

Fab fragment in complex with small molecule hapten, crystal form-1

Released:
DOI: 10.2210/pdb2jb5/pdb
PDB entry 2jb5 coloured by chain, front view.
PDB entry 2jb5 coloured by chain, side view.
PDB entry 2jb5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded Fab dimer interface.
Hillig RC, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun DI, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
J Mol Biol 377 206-19 (2008)
PMID: 18241888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209620 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FAB FRAGMENT MOR03268 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 244 amino acids
Theoretical weight: 26.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin lambda constant 2 Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 22.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DOY2 (Residues: 1-104; Coverage: 98%)
Gene name: IGLC2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand T5C 1 x T5C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I222
Unit cell:
a: 71.949Å b: 98.48Å c: 153.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.272
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
Rich et al. (2010)
1 more

2 mentions without citation

Computational design of an epitope-specific Keap1 binding antibody using hotspot residues grafting and CDR loop swapping.
Liu et al. (2017)
1 more