› 2jdw

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE AND MECHANISM OF L-ARGININE: GLYCINE AMIDINOTRANSFERASE: A MITOCHONDRIAL ENZYME INVOLVED IN CREATINE BIOSYNTHESIS

Released:
DOI: 10.2210/pdb2jdw/pdb
PDB entry 2jdw coloured by chain, front view.
PDB entry 2jdw coloured by chain, side view.
PDB entry 2jdw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure and mechanism of human L-arginine:glycine amidinotransferase: a mitochondrial enzyme involved in creatine biosynthesis.
Humm A, Fritsche E, Steinbacher S, Huber R
EMBO J 16 3373-85 (1997)
PMID: 9218780

Function and Biology Details

Reaction catalysed: 
L-arginine + glycine = L-ornithine + guanidinoacetate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine amidinotransferase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 48.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50440 (Residues: 1-423; Coverage: 100%)
Gene names: AGAT, GATM
Structure domains: L-arginine/glycine Amidinotransferase; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P43212
Unit cell:
a: 83.6Å b: 83.6Å c: 200.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 not available
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Creatine and creatinine metabolism.
Wyss et al. (2000)
10 more

1 mention without citation

Genetic variants in genes involved in creatine biosynthesis in patients with severe obesity or anorexia nervosa.
Rajcsanyi et al. (2023)